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Light micrograph of Kupffer cells stained with trypan blue. Kupffer cells are macrophages that are localised in the lumen of the liver sinusoids and attached to the sinusoidal endothelial cells. Trypan blue is a vital stain that can be used in the body. Circulating trypan blue is phagocytosed by macrophages and, in the liver, by Kupffer cells, which become stained blue.

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