This is a super-resolution LM image taken by Hiro Hakozaki and Masa Hoshijima of NCMIR. The image contains highlighted calcium channels in cardiac muscle using a technique called dSTORM. The microscope used in the NCMIR lab was built by Hiro Hakozaki. Cardiac muscle (heart muscle) is involuntary striated muscle that is found in the walls and histological foundation of the heart, specifically the myocardium. Cardiac muscle is one of three major types of muscle, the others being skeletal and smooth muscle. dSTORM utilizes the photo-switching of a single fluorophore. In dSTORM, fluorophores are embedded in an oxidizing and reducing buffer system (ROXS) and fluorescence is excited. Sometimes, stochastically, the fluorophore will enter a triplet state, in which it is susceptible to the reducing components in the buffer. The fluorophore is reduced into a long-lived radical state, which is dark for several seconds.