Edward Augustus Holyoke (August 1, 1728 - March 31, 1829) was an American educator and physician. He opened a medical practice in 1748 and practiced for 73 more years, until retiring in 1821. He was a pioneer in the advancement of smallpox vaccinations. Holyoke was a charter member of the American Academy of Arts and Sciences and a founder and president of the Massachusetts Medical Society. He died in 1829 at the age of 100, surpassing the average life expectancy of the time by fifty years.